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Experimental results demonstrating intracellular imaging of host-pathogen interactions have just been published in the Journal of Biophotonics:
Iain Robinson, Michael Andreas Ochsenkühn, Colin J. Campbel, Gerard Giraud, William J. Hossack, Jochen Arlt and Jason Crain, Intracellular imagng of host-pathogen interactions using combined CARS and two-photon fluorescence microscopies, Journal of Biophotonics 3, 138 - 146 (2010) DOI:10.1002/jbio.200910054
The team of researcher led by Jason Crain used a of combined coherent anti-Stokes Raman scattering (CARS) and two-photon fluorescence microscopies to image fibroblast cells infected by cytomegalovirus. CARS is used to image the host cell membrane, lipid droplets and morphology of the nucleus. Using a genetically modified virus strain expressing the green fluorescent protein also enables two-photon imaging of the same cells to reveal the location, nature and extent of viral protein expression.